84-1196-24　Spinophilin (Neurabin-II, PPP1R9B) 100ug　S5525A

特徴

• PPP1R9B is a widely expressed intracellular protein that is found in both cytoplasm and nucleus.
• Although its predicted MW is 89kD, it runs anolamously at 120-140kD in SDS-Page.
• PPP1R9B is associated with actin polymerization, particularly in cells such as neurons and NK cells.
• In NK cells, it orchestrates actin organization around the immunological synapse, allowing for targeted release of cytotoxic granules.
• Human PPP1R9B is 815aa in length.
• It contains one actin-binding region aa1-154, a PP1 binding domain aa415-492, a PZD domain aa493-583 and a coiled-coil region aa647-815.
• PPP1R9B is reported to form homotrimers and homotetramers.
• There is one isoform variant that shows an Ala insertion after both Ala158 and Ala165, coupled to a premature truncation after Arg576.
• Over aa291-414, human PPP1R9B shares 91aa identity with mouse PPP1R9B.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.