Semaphorin 4G is a 92kD (predicted), class IV member of the semaphorin family of proteins.
It is expressed by select cell types, including oligodendroglia and Purkinje neurons, and likely acts as a chemorepellent by binding to B-type plexins.
Mature mouse Sema4G is a type I transmembrane protein that is 820aa in length.
It contains a 656aa extracellular region aa18-673 that is characterized by one Sema domain aa35-503, a PSI region aa505-556 and an Ig-like C2-type domain aa565-648.
The 143aa cytoplasmic region contains a poly-proline segment and a Ser phosphorylation site.
Over aa 18-673, mouse Sema4G shares 91-97aa identity with human and rat Sema4G, respectively.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
Specificity：Recognizes mouse Semaphorin 4G. Species sequence homology: Recombinant human Semaphorin 4G, recombinant mouse (1%), Semaphorin 4B, Semaphorin 4C, and Semaphorin 4F (20%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant corresponding to aa18-673 from mouse Semaphorin 4G expressed in NS0 cells (Q9WUH7).
