SCTR is a 51-62kD member of the G-protein coupled receptor 2 family.
It is found on alveolar epithelium, bile duct epithelium, pancreatic exocrine duct epithelium, and stomach plus duodenal mucosal epithelium.
Mature human SCTR is a 418aa 7-transmembrane glycoprotein aa23-440.
It contains a 121aa N-terminal extracellular region aa23-143 plus a 48aa C-terminal cytoplasmic domain aa393-440.
There is at least one splice variant that shows a deletion of aa66-101.
SCTR forms homodimers and homooligomers, and heterodimerizes with almost all family B GPCRs (VPAC1; VPAC2; GLP1R; GHRHR; etc.).
Splice form of human Secretin Receptor with deletion of exons 3 and 4 was found expressed in pancreatic adenocarcinomas and cholangiocellular carcinomas, but not in gastrinomas or nonneoplastic pancreas or liver specimens.The deletion in this splice form is predicted to lead to a frame-shift, early truncation, and total absence of transmembrane segments in the receptor protein, whereas the leader sequence responsible for trafficking of the receptor to the cell membrane is preserved.
This encoded a 111-residue peptide with its first 43 residues identical to wild-type receptor; but, subsequent to a shift in coding frame and early truncation, the next 68 residues were unique in the transcriptome/proteome.
Our antibody was raised against this unique 68aa sequence resulting from putative frame-shift.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
Specificity：Recognizes human Secretin R delta 3/4.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to human Secretin R delta 3/4 Splice variant (1-3) expressed in E. coli.
