Mouse S100A16 is a 15-18kD member of the S100 family of calcium-binding proteins.
It is found in both cytoplasm (when Ca is high) and nucleus (when Ca is low), and its expression appears to be restricted to astrocytes found in select areas of the brain.
S100A16 is 124aa in length.
Based on human, it will contain an N-terminal non-Ca-binding EF-hand domain aa12-47, a C-terminal Ca-binding EF-hand domain aa54-89, and a putative FGF-1 binding site aa91-94.
There is one potential myristoylation site aa54-59 and a phosphorylation site at Ser124.
S100A16 will homodimerize, and perhaps form higher order oligomers.
Full length mouse S100A16 shares 95aa identity with rat S100A16.
Relative to human S100A16, mouse S100A16 possesses a 21aa Glu/Gln-rich insertion after Glu98 that reduces the shared aa homology between mouse and human S100A16 to 72aa identity.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
Specificity：Recognizes mouse S100A16. Species sequence homology: Recombinant human S100A16 (30%), recombinant mouse S100A4, recombinant human S100A2, and S100A10 (1%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：Recombinant corresponding to aa2-124 from mouse S100A16 expressed in E. coli (NP_080692).
