R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27kD secreted protein that shares 40% aa identity with three otherR-Spondin family members (1, 2).
All R-Spondins regulate Wnt/b-catenin signaling, but have distinct expression patterns (1-3).
Like other R-Spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (aa34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP-1) motif (aa147-207) and a region rich in basic residues (aa211-263).
Only the furin-like domains are needed for b-catenin stabilization (2, 4).
A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (5).
Potential isoforms of 200-236aa have an alternate, shorter N-terminus or are missing aa146-208, respectively (6).
Over aa21-263, human R-Spondin 1 shares 89%, 87%, 92%, 91%, 91% and 89aa identity with mouse, rat, equine, canine, caprine and bovine R-Spondin 1, respectively.
R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 5).
In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (7, 8).
Disruption is also associated with palmoplantar keratosis (7, 8).
Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (9).
Injection of recombinant R-Spondin 1 in mice causes activation of b-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (9, 10).
R-Spondin 1 regulates Wnt/b-catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization (11).
Reports differ on whether R-Spondin 1 binds LRP-6 directly (11-13).
Applications:Suitable for use in Neutralization.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
Specificity：Recognizes human R-Spondin 1. Species sequence homology: Recombinant mouse R-Spondin 1 (6%), recombinant human R-Spondin 2, rhR-Spondin 3, and rhR-Spondin 4 (1%).
Isotype：IgG
Calc Applications Abbrev：N
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa31-263 from human R-Spondin 1 expressed in CHO cells.
