RAMP2 is a 20kD member of the RAMP family of proteins.
It is expressed on cardiomyocytes, vascular smooth muscle cells and endothelium, and interacts with CRLR to form a receptor complex for adrenomedullin (AM).
AM induces vasodilation on AM1 receptor expressing cells.
Mature mouse RAMP2 is a 145aa type I transmembrane glycoprotein that contains a 115aa extracellular domain (ECD) aa45-159 and a nine aa cytoplasmic region.
Although the ECD contains no typical structural motifs, based on human, aa100-106 are critical for AM binding.
Over aa45-159, mouse RAMP2 shares 57-83aa identity with human and rat RAMP2, respectively.
Applications:Suitable for use in Flow Cytometry and Western Blot.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 2.5ug labels 10e6 cells Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse RAMP2. In direct ELISA crossreacts ~25% with recombinant human RAMP2 and <1% with recombinant human RAMP1.
Isotype：IgG
Calc Applications Abbrev：FC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to aa45-159 from mouse RAMP2 expressed in E. coli (Q9WUP0).
