PGC-1a is a 97-120kD member of the PGC-1 family of proteins.
It is expressed in select cell types, including brown adipocytes, skeletal muscle and hepatocytes.
PGC-1a participates in both RNA processing and transcriptional coactivation in conjunction with multiple nuclear hormone receptors such as PPARg, RAR and TR.
Human PCG-1a is 798aa in length.
It contains an LxxLL nuclear receptor binding motif aa144-148, one PPAR-g interaction domain aa293-339, two NLSs and an RNA binding/processing region aa566-710.
PGC-1a activity is regulated by phosphorylation.
AMPK is known to phosphorylate Thr178 and Ser539, promoting cotranscriptional activity.
Conversely, Akt-mediated phosphorylation at Ser571 is reported to downregulate PGC-1a activity.
This latter effect is achieved by an initial Ser571 phosphorylation, followed by GCN5 binding and subsequent PCG-1a acetylation that promotes PGC-1a dissociation from target gene promoters.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml using HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line treated with recombinant human IGF-1Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human PGC1 alpha, when phosphorylated at S571.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Phosphopeptide containing the human PGC1a S571 site.
