Perilipin is a 62kD constitutively phosphorylated protein that belongs to the PAT (Perilipin/Adipophilin/TIP47) family of molecules.
Found primarily in mature adipocytes, perilipin-1 interacts with lipid-coat proteins at the edge of the fat droplet and blocks lipase activity.
Upon phosphorylation, perilipin-1 becomes a 65-67kD molecule that likely promotes the dispersion of docking molecules and provides a scaffold for lipase at the lipid droplet surface.
Human perilipin-1 is 522aa in length.
It contains a lipid droplet targeting region aa233-364 that contains a polyGlu segment aa307-316.
Phosphorylation at the N-terminus is necessary for lipase interaction with lipid.
One 50-54kD human splice variant (perilipin-B) is reported that shows an 85aa substitution for aa404-522.
This apparently interacts with cell membrane triglycerides.
Over aa8-145, human perilipin-1/A shares 97aa identity with mouse perilipin-1.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
Specificity：Recognizes human Perilipin.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa8-145 from human Perilipin expressed in E. coli (O60240).
