PAX7 is a 57kD member of the paired homeobox family of transcription factors.
It is expressed in select neurons (in rodent) and skeletal muscle satellite cells, where, in the latter, it promotes both a muscle stem cell state (PAX7+ desmin-cells) and a lineage commitment state (PAX7+ desmin+ cells).
PAX7 forms heterodimers with PAX3, and interacts with the regulatory regions of the Myf5 locus.
Human PAX7(A) is 520aa in length.
It contains an N-terminal paired box DNA-binding domain (aa34-163), a homeodomain (aa218-275) and a C-terminal poly-alanine region (aa340-346).
Multiple splice forms of PAX7 exist.
There is a 54kD isoform B that contains a 38aa substitution for aa468-520, plus a second isoform that shows a deletion of Gly151 and Leu152.
There is also a chromosomal rearrangement that fuses aa30-385 of PAX7 to aa211-265 of FOXO1A/FKHR.
Over aa1-196, human PAX7 shares 86-96aa identity with human PAX3 and mouse PAX7.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
Specificity：Recognizes human Pax7. Species sequence homology: recombinant human Pax3 (50%), rhPax1, rhPax6 (4%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa1-196 from human Pax7 expressed in E. coli (P23759).
