PILR-a (paired immunoglobulin-like, type 2 receptor alpha) is a 44-50kD type I transmembrane (TM) paired receptor glycoprotein that belongs to the Ig Superfamily.
It is expressed by monocytes, macrophages, CD14+CD1a- DC and retinal pigment cells, and is known to bind to CD99 and PANP.
PILR-a acts as a receptor for HSV and serves as a negative immunomodulator that contains an ITIM.
Mature human PILR-a is 284aa in length.
It contains one V-type Ig-like domain in its extracellular region (aa32-150), and two ITIMs in its cytoplasmic domain (aa267-272 and 296-301).
There are multiple potential splice variants.
One is TM and possesses a 35 aa substitution for aa264-303, while others are soluble, and show a deletion of aa152-224 that may be coupled to the 35aa substitution noted above, or simply exhibit a 24aa substitution for aa152-303.
Over aa20-196, human PILR-a shares only 42aa identity with mouse PILR-a, and 89aa identity with human PILR-b.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 2.5ug labels 10e6 cellsOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
Specificity：Recognizes human PILR-a.
Isotype：IgG
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa20-196 from human PILR-a expressed in NSO cells (Q9UKJ1).
