NeuroD2 is a 41kD nuclear member of the neuroD family of transcription factors.
It is expressed in developing and mature neurons such as hippocampal granular neurons, and acts (in part) to repress factors that would otherwise block multipotential cell commitment to a neuronal lineage.
NeuroD2 is presumed to act as a heterodimer with other bHLH transcription factors.
Human NeuroD2 is 382aa in length.
It contains a poly-Glu region aa82-91, an NLS aa107-113, a DNA-binding HLH domain aa119-178 and a poly-Gly segment aa282-285.
Based on rat studies, human NeuroD2 will undergo variable phosphorylation in the C-terminal region.
Over aa174-382, human and mouse NeuroD2 possess identical aa sequences.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
Specificity：Recognizes human NeuroD2. Species sequence homology: Recombinant human NeuroD1 (20%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa174-382 from human NeuroD2 expressed in E. coli (Q15784).
