NALP3 is a cytoplasmic 115-130kD member of the NLRP family of molecules.
It is selectively expressed, being found in stratified squamous and transitional epithelium, Hassell's corpuscles, neutrophils, dendritic cells and T cells.
NALP3 promotes IL-1b and IL-18 maturation by activating caspase-1.
It does so by forming a NALP3 inflammasome comprised of NALP3, ASC and caspase-1.
Bacterial RNA and cell wall peptidoglycan can bind to NALP3, promoting (potentially) ATP binding, NALP3 oligomerization, and caspase activation.
Human NALP3 is 1036aa in length.
It contains an N-terminal DAPIN domain (aa1-93), a NACHT domain (aa220-536), and seven consecutive LRRs (aa740-991).
Alternate splice forms are reported.
Either individually, or in combination, there can be deletions of aa836-892, 721-777, and a premature truncation after Gly719 that generates a 70kD isoform.
Over aa3-223, human NALP3 shares 76aa identity with mouse NALP3.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
Specificity：Recognizes human NALP3. Species sequence homology: recombinant human NALP1 and rhNALP2 (1%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa4-223 from human NALP3 expressed in E. coli (Q96P20).
