NALP1 is a 160-170kD member of the NLRP family of molecules.
It is expressed in dendritic cells, neutrophils, T and B cells, alveolar macrophages, permatogonia, neurons, plus intestinal columnar epithelium, and is found in both cytoplasm and nucleus.
NALP1 promotes apoptosis plus IL-1b and IL-18 maturation by activating caspase-1 and -5.
It does so by forming a 700kD inflammasome comprised of NALP1, ASC plus caspase-1 and -5.
Bacterial wall peptidoglycan binds to NALP1, promoting ATP binding, NALP1 oligomerization, and caspase activation.
Human NALP1 is 1473aa in length.
It contains an N-terminal DAPIN domain (aa1-92), a NACHT domain (aa328-637), seven consecutive LRRs (aa704-1236) and one CARD region (aa1374-1463).
Alternate splice forms exist that range in size from 70-150kD.
Either individually, or in combination, there can be a 19aa substitution for either aa1353-1473 or aa1368-1472, a deletion of aa91-260, 958-987, 1262-1305, and a 43aa insert after aa785.
Over aa1-323, human NALP1 shares less than 20aa identity with mouse NALP1.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
Specificity：Recognizes human NALP1. Species sequence homology: recombinant human NALP2, rhNALP3 (2%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa1-323 (aa155) from human NALP1 expressed in E. coli (Q9C000).
