MFNG is a 52-55kD member of the glucosyltransferase 31 family.
It is a Golgi membrane protein that transfers N-acetylglucosamine to an O-linked fucose residue on Notch.
Activity on Notch increases Delta-1 induced signaling while suppressing Jagged-1 signaling.
MFNG is found in fetal pancreatic endocrine progenitor cells and immature ventricular zone neurons.
Human MFNG is a 321aa type II transmembrane protein.
It contains a short 7aa cytoplasmic region, plus a 294aa luminal domain (aa28-321).
There are two potential splice variants, one that shows a 15aa substitution for aa104-321, and another that contains a three aa substitution for aa86-102.
Over aa37-321, human MFNG shares 85% aa identity with mouse MFNG.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunoprecipitation: 25ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute with sterile PBS to 200ug/ml.
Specificity：Recognizes human MFNG. Species sequence homology: mouse (85%).
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa37-321 from human MFNG expressed in NSO cells (O00587).
