84-1195-91　Lipoprotein Receptor-related Protein 6, Low Density, phosphorylated (S1490) (LRP6, FLJ90062, FLJ90421) 100ug　L2601-06J1

特徴

• LRP6 (Low-density lipoprotein receptor-related protein 6) is a 200-210kD member of the LDLR family of proteins.
• It is widely expressed, and serves as a coreceptor for both Wnt and parathyroid hormone.
• In the Wnt system, LRP6 associates with select Fzd multipass receptors; in the PTH system, LRP6 complexes with PTHR1.
• Mature human LRP6 is a 1594aa type I transmembrane glycoprotein.
• It contains a 1351aa extracellular region (aa20-1370) plus a 220aa cytoplasmic domain (aa1394-1613).
• The cytoplasmic domain contains two palmitoylation sites, one ubiquitination residue, and multiple phosphorylation motifs.
• aa1490 is a key residue that impacts multiple activities.
• It undergoes both constitutive and receptor activation-induced phosphorylation.
• At least three enzymes likely act at this site, including PKA (associated with PTHR1), GSK-3 (associated with Fzd) and Cyclin Y/PFTK1 (associated with G2/M of the cell cycle).
• In general, Ser1490 phosphorylation results in b-catenin stabilization, followed by either gene transcription or b-catenin binding to centrosomes.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.