LRP-1B is a 600-620kD member of the LDLR family of proteins.
It is expressed on vascular smooth muscle cells and likely neurons, and binds multiple molecules,including uPA,PAI-1,PDGFRb, uPAR, and Pseudomonasexotoxin.Itisconsideredatumor suppressor, and appears to block the generation of plasmin.
Mature human LRP-1B is a 4579aa type I transmembrane (TM) glycoprotein.
It contains a 4424aa extracellular domain (ECD) aa21-4444 plus a 132aa cytoplasmic region aa4468-4599.
In the ECD, there are 32 LDLR class A repeats, 14 EGF-like repeats, and 36 LDLR class B repeats that show an interspersing pattern.
In the cytoplasmic region, one NLS aa4468-4474 and two endocytosis signal motifs aa4492-4562 are found.
LRP-B1 is cleaved in the Golgi by furin after Arg3957 to create a noncovalent heterodimer consisting of a 515kD ECD, and an 85-95kD TM segment.
Either uncleaved LRP-1B, or the dissociated heterodimer can undergo additional processing, leading to cleavage of the 90kD TM segment after Lys4435.
This creates a 21kD TM C-terminal fragment that can undergo further g-secretase processing to generate a cytoplasmic, 18kD peptide that translocates to the nucleus.
Over aa21-244, human LRP-1B shares 81% aa identity with mouse LRP-1B.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml on human liver tissue lysatesOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human LRP-1B. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) LRP‑1, rhLRP‑4, rhLRP‑5, and rhLRP‑6 is observed.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Leu21-Glu244 from human LRP-1B expressed in E. coli.
