IL-23 p19 is an 18-20kD (estimated) member of the IL-6 superfamily of molecules.
It is disulfide-bonded to p40 to form IL-23.
IL-23 is secreted by immune-related cell types such as keratinocytes, dendritic cells, macrophages, microglia and monocytes, often following TLR stimulation.
It appears to drive Th17 cell development by inhibiting T-bet and FoxP3 production, and to reduce IL-12-mediated IFN-g production by CD4+, CD8+ and NK cells.
Based on human, mature feline IL-23 p19 is 160aa in length.
It is a a-helical molecule that utilizes Cys80 to form an interchain disulfide bond with IL-12 p40.
IL-23 p19 does not appear to be released unless dimerized to p40.
There are no potential N-linked glycosylation sites on p19.
Mature feline IL-23 p19 aa26-185 shares 87aa identity with human IL-23 p19.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：feline
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
Specificity：Recognizes feline IL-23 p19. Species Crossreactivity: human. Species sequence homology: Recombinant canine IL-23 p19 (60%), recombinant mouse IL-23 p19 (30%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Fe Hu
Immunogen：Recombinant corresponding to aa26-185 from feline IL-23 p19 expressed in E. coli (ABB01676).
