Two distinct receptors bind the pleiotropic cytokines IL-1a and IL-1b.
The IL-1 Receptor I (IL-1 RI) is an 80kD transmembrane protein expressed predominantly in T cells, fibroblasts, and endothelial cells.
IL-1 Receptor II is a 68kD protein that is found on B lymphocytes, neutrophils, monocytes, large granular leukocytes, and endothelial cells.
Both receptors are members of the immunoglobulin superfamily and show ~28% sequence identity in their extracellular domains.
The two receptors do not heterodimerize into a receptor complex.
Soluble recombinant IL-1 Receptor I is a potent antagonist of IL-1 action.
Applications:Suitable for use in FLISA, Western Blot and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 10ul/10e6 using HUVEC human umbilical vein endothelial cellsOptimal dilutions to be determined by the researcher.
Absorption:620-650nm
Emission:660-670nm
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human Interleukin 1 Receptor Type I.
Isotype：IgG
Calc Applications Abbrev：FC FLISA WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Interleukin 1 Receptor Type I expressed in Sf-21 cells.
