84-1195-84　HSPC142 (BRCA1-A Complex Subunit MERIT40, Chromosome 19 Open Reading Frame 62, C19orf62, FLJ20571, Mediator of RAP80 Interactions and Targeting Subunit of 40kD, MERIT40, New Component of the BRCA1-A Complex, NBA1) 100ug　H7680-02C

特徴

• MERIT40 is a 40kD member of the MERIT40 family of proteins.
• It is ubiquitously expressed, and participates in the DNA damage response.
• At sites of double strand breaks, histones are phosphorylated, ubiquitinated, and deubiquitinated, with repairs performed by a sequentially formed complex that contains BRCA1, Rap80 and BRE.
• MERIT40 apparently serves as a scaffold protein that maintains the integrity of the complex.
• Human MERIT40 is 329aa in length.
• It contains one VWF-A domain aa95-298 and six utilized Ser/Thr phosphorylation sites.
• There are three potential splice variants.
• One shows a 13aa substitution for aa234-329, a second possesses a 105aa substitution for aa263-329, while a third shows a 37aa substitution for aa1-115, coupled to a deletion of aa263-329.
• Over aa182-329, human MERIT40 shares 95aa identity with mouse MERIT40.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.