84-1195-83　HS6ST3 (Heparan sulfate 6-O-sulfotransferase 3) 100ug　H7520-02

特徴

• HS6ST3 (Heparin sulfate 6-O-sulfotransferase 3) is a 60-62kD member of the sulfotransferase 6 family of enzymes.
• It is one of three 6-O sulfotransferase isoforms, all of which essentially perform the same function, but all of which are the products of distinct genes.
• HS6ST3 is known to sulfate the #6 position of glucosamine sulfate that is linked to a 2-O-sulfated iduronic acid in heparin sulfate.
• The molecule shows a temporal expression pattern, being found in postnatal cerebellar Purkinje cells and cerebral cortical pyramidal cells in layers II/III and V.
• Mouse HS6ST3 is a 470aa type II transmembrane glycoprotein.
• It contains a short four aa cytoplasmic N-terminus coupled to an extended 443aa lumenal domain (aa28-470).
• The lumenal domain contains a stem region (aa28-83) that mediates oligomerization and Golgi localization, plus a sulfotransferase enzyme domain (aa142-409).
• Cleavage within the stem region by b-secretase generates a soluble form of HS6ST3.
• Over aa28-470, mouse HS6ST3 shares 99-95aa identity with rat and human HS6ST3, respectively.
• Applications:Suitable for use in Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.