At the G2/M transition, H4 is monomethylated on Lys20 and possibly contributes to transcription initiation/elongation.
As the cell moves into the M/G1 phase, Lys20 becomes either dimethylated or trimethylated, contributing to DNA repair and telomere maintenance, respectively.
H4 is also known to be phosphorylated on aa2-48, plus aa52-89, and acetylated on at least nine sites.
Human histone 4 is 103aa in length.
Full length histone 4 is identical in aa sequence to mouse histone 4, and 78aa identical to Tetrahymena histone 4 over aa2-20 used for immunization.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunocytochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
