84-1195-78　FHL1 (Four and a Half LIM Domains 1, FHL-1, FHL1A, FHL1B, FLH1A, bA535K18.1, KyoT, LIM Protein SLIMMER, MGC111107, RBP Associated Molecule 14-1, RAM14-1, Skeletal Muscle LIM Protein 1, SLIM1, SLIM-1, SLIM, XMPMA) 100ug　F4149-77E1

特徴

• FHL1 is a 36kD member of the four-and-a half class of the LIM domain-only protein family.
• It is highly expressed in myocardium and skeletal muscle, and is now known to bind NFATc1, promoting muscle hypertrophy.
• It is also noted to be a RIP140 binding partner that inhibits estrogen receptor signaling.
• Human FHL1 is 323aa in length.
• It contains one C4-type Zn-finger region (aa7-31), three distinct LIM domains (aa40-77; 101-158; 162-212) and a bipartite NLS (aa231-246).
• There is one 32kD splice variant that shows a 50 aa substitution for aa231-323.
• This creates a fourth LIM domain.
• There are also alternative start sites aa29-16 upstream of the standard site, a deletion of aa168-296, and an 11 aa substitution for aa81-91.
• Over aa93-166, human FHL1 is 97aa identical to mouse FHL1.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.