DCBLD2, was identified through its up-regulation in a highly metastatic lung cancer cell line.
DCBLD2 has structural similarities to neuropilins, VEGF receptors, and semaphorins, and is thought to play a role in cell motility and metastasis.
SEMA4B is a receptor for DCBLD2/ESDN/CLCP1 and this interaction may be involved in regulating cancer cell metastasis.
Applications:Suitable for use in Western Blot and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5ug/ml using P19 mouse embryonal carcinoma cell lineFlow Cytometry: 2.5ug labels 10e6 cells using NCI-H460 human large cell lung carcinoma cell lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS
Specificity：Recognizes human ESDN. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：FC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to Gln67-Ala528 from human ESDN expressed in NS0 cells
