Applications:Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 0.25ug/ml labels 10e6 of COLO 205 human colorectal adenocarcinoma cell line and D3 mouse embryonic stem cell lineWestern Blot: 0.1ug/mlImmunohistochemistry: 1-15ug/ml using immersion fixed frozen sections of embryonic mouse brain and mouse embryo and paraffin sections of human esophageal squamous cellImmunocytochemistry: 5-15ug/ml of immersion fixed MDA-MB-468 human breast cancer cel lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse EphB2. Species Crossreactivity: human. In Western Blots, this antibody shows ~5% crossreactivity with rrEphB1, rmEphA8, rmEphA6, rmEphB6, rmEphA3, rmEphA4, rmEphA7, rrEphA5, rhEphA1, rmEphA2 and rmEphB3.
Isotype：IgG
Calc Applications Abbrev：FC IC IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to Val27-Lys548 of mouse EphB2 expressed in NS0 cells.
