84-1195-70　DPT (Dermatopontin, Tyrosine-rich Acidic Matrix Protein, TRAMP) 100ug　D3212-51C

特徴

• Dermatopontin, is a widely expressed noncollagenous protein component of the extracellular matrix (1,2).
• Mature human Dermatopontin shares 96%, 92%, and 92% aa sequence identity with bovine, mouse, and rat Dermatopontin, respectively.
• It is a 22kD molecule that is tyrosine sulfated but not glycosylated (3,4).
• Dermatopontin contains three disulfide bonded loop structures that enclose conserved hexapeptide motifs (5).
• It accelerates collagen fibril formation in vitro, and Dermatopontin deficient mice exhibit altered collagen matrix deposition and organization (6-8).
• Dermatopontin is downregulated in fibrotic growths such as leiomyoma and scar tissue (9,10).
• It binds both TGF-b and the proteoglycan decorin, interactions that can increase the bioavailability of TGF-b (11,12).
• Dermatopontin promotes bone mineralization under the control of the vitamin D receptor and inhibits BMP-2 effects on osteoblast precursors (13,14).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/ml using lysates of human heart tissueOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.