84-1195-69　［取扱停止］DARC (Duffy Antigen/Chemokine Receptor, Fy Glycoprotein, Glycoprotein D, Plasmodium Vivax Receptor) 100ug　D1101-01C

特徴

• DARC (Duffy Antigen Receptor for Chemokines; also CD234) is a 40-46kD glycoprotein member of the Duffy family of silent heptahelical chemokine receptors.
• It is expressed in liver and on select neurons, erythrocytes and the endothelium of postcapillary venules.
• Unlike traditional chemokine receptors, DARC cannot signal through G-proteins as it lacks a DRYLAIVHA cytoplasmic motif.
• DARC has three potential functions: first, it binds circulating inflammatory-type chemokines, serving as a repository for future chemokine release; second, it acts as a vehicle by which chemokines are transported from the abluminal to the luminal side of endothelium; and third, it complexes with signal-transducing chemokine receptors to create a nonsignaling heterodimer.
• Mouse DARC is 334aa in length.
• It contains a 62aa N-terminal extracellular region, and a 28aa C-terminal cytoplasmic tail.
• There is one potential splice variant that shows a 42aa substitution for aa133-334.
• Collectively, over the four extracellular domains (aa1-62, 115-127, 186-205, 264-285), mouse DARC shares 52-75aa identity with human and rat DARC, respectively.
• Applications:Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilution:Flow Cytometry: 0.25ug per 10e6 cellsImmunohistochemistry: 5-15ug/mlWestern Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.