Cystatin D is a 17kD member of the type 2 cystatin family of proteins.
It is expressed in salivary gland, skin, prehypertrophic and hypertrophic chondrocytes.
Within the context of endochondral ossification, it may both induce chondrocyte apoptosis and initiate the process of cartilage calcification.
Although Cystatin D contains a key cystatin motif (Gln-x-Val-x-Gly), Cystatin D does not appear to inhibit cysteine proteases.
Mouse Cystatin D is 148aa in length.
It contains one cystatin-like domain aa39-145.
There are two potential splice variants.
One shows a deletion of aa84-121, while a second shows a Val substitution for aa122-148.
Full-length mouse Cystatin D shares 78aa identity with rat Cystatin D.
There appears to be no direct human counterpart to mouse Cystatin D, as both human cystatin-C and -D share less that 40% aa identity with mouse Cystatin D.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 2ug/ml using mouse salivary gland tissue
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse Cystatin D. In direct ELISAs, less than 4% cross-reactivity with recombinant human (rh) Cst1, rhCst2, and rhCst3 is observed.Recombinant human, Cst1, rhCst2, and rhCst3 (4%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Asn30-Phe148 from mouse Cystatin D, expressed in NSO cells.
