84-1195-67　Contactin 6 (Neural recognition molecule NB-3, hNB-3, Contactin-6 precursor, MGC133256, NB3, NB-3) 100ug　C7865-25C

特徴

• Contactin-6, is a 130kD member of the TAG/F3 subgroup of Contactin GPI-linked neuronal adhesion proteins (1,2).
• Mature mouse Contactin-6 consists of six immunoglobulin-like domains, a flexible linker region, four fibronectin type III domains, and a GPI anchor (3-5).
• Mouse Contactin-6 shares 90-96aa sequence identity with human and rat Contactin-6, respectively.
• Alternate splicing generates an isoform with a 17aa deletion in the first Ig-like domain (3).
• Contactin-6 expression is upregulated postnatally in the cerebellum, brainstem, and hippocampus, and it shows differential expression within various structures of the brain (3,5-7).
• In the cerebellum, it is presynaptically localized at synapses between excitatory glutamatergic parallel fibers and Purkinje cells (7).
• Contactin-6 associates in cis with CHL-1 and in trans with Notch-1 on oligodendrocytes (6,8).
• Its binding to Notch-1 triggers translocation of the Notch intracellular domain to the nucleus and promotes oligodendrogenesis (6).
• Contactin-6 function is important in the postnatal development and function of the cerebellum, as shown in knockout mice which exhibit poor motor coordination (5,7).
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.