Contactin-4, is an axonal cell adhesion molecule (AxCAM) that belongs to thecontactin family, a subfamily of the Ig superfamily (1, 2).
The contactin family comprises six members (CNTN1/F3, CNTN2/TAG-1, CNTN3/BIG-1, CNTN4/BIG-2, CNTN5/NB-2 and CNTN6/NB-3) that are characterized by the presence of six Ig like domains, four fibronectin type III-like repeats, and a glycosylphosphatidylinositol (GPI)-anchoring domain (1-4).
Contactins are membrane-anchored proteins that can be released as soluble proteins by GPI-specific phospholipase D and are able to promote neurite outgrowth in their soluble form (2).
Potential 1026, 705-498aa isoforms of mouse CNTN4 have been described (1, 5).
Only the longest isoform includes the C-terminal GPI anchoring sequence.
It shares 97% aa identity with rat and 95aa identity with human, equine and bovine CNTN4 in its mature 981aa form.
It also shares 42-64% aa identity with other CNTN family members, showing highest identity with CNTN3.
CNTN4 is expressed throughout the brain, but minor amounts are also detected in small intestine, thyroid, uterus and testis (2, 4).
Family members display overlapping but distinct expression patterns in rat brain and are suggested to influence the formation and maintenance of specific neuronal networks (2).
In the olfactory bulb, CNTN4 is expressed in specific sensory neurons in a mosaic pattern that is closely correlated with odorant receptor choice, and is thought important for odor mapping (6, 7).
In humans, disruption of CNTN4 has been implicated in the 3p deletion syndrome characterized by developmental and growth delay, and in autism spectrum disorder (8-10).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse Contactin-4. Species sequence homology: Recombinant human Contactin-4 (35%), and Contactin-5, recombinant mouse Contactin-3 and Contactin-6 (1%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：Recombinant corresponding to aa19-999 from mouse Contactin-4 expressed in CHO cells (Q69Z26).
