CD302 is a 31-36kD member of the C-type lectin domain family of proteins.
It is expressed on cells involved in phagocytosis, including neutrophils, monocytes, dendritic cells and macrophages.
CD302 is suggested to facilitate phagocytosis, and participate in cell adhesion.
Mature human CD302 is a 210aa type I transmembrane glycoprotein.
It contains a 146aa extracellular domain (ECD) aa23-168 plus a 43aa cytoplasmic region.
The ECD possesses a C-type lectin domain aa32-152 that apparently cannot bind carbohydrate.
CD302 has also been detected in dendritic cells as the C-terminal portion of a 215kD fusion protein that involves DEC205.
CD302 contributes aa23-232 to the fusion protein.
There is one potential alternative start site at Met63.
Over aa23-168, human CD302 shares 82aa identity with mouse CD302.
Applications:Suitable for use in Western Blot and Flow Cytometry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml in HL‑60 human acute promyelocytic leukemia cell lineFlow Cytometry: 2.5ug labels 10e6 cells in human peripheral blood monocytes Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human CD302. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse CD302 is observed.
Isotype：IgG
Calc Applications Abbrev：FC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala20-Asn167 from human CD302 expressed in NSO cells.
