The high-affinity IFN-G receptor complex is made up of two type I membrane proteins, IFN-G R1 (IFN-G Ra) and IFN-G R2 (IFN-G Rb).
Both proteins are members of the type II cytokine receptor family and share ~52% overall sequence identity.
IFN-G R1 is the ligand-binding subunit that is necessary and sufficient for IFN-G binding and receptor internalization.
IFN-G R2 is required for IFN-G signaling but does not bind IFN-G by itself.
Human IFN-G R1 cDNA encodes a 499aa residue protein with a 17aa signal peptide, a 228aa extracellular domain, a 23aa transmembrane domain, and a 221aa intracellular domain.
Human and mouse IFN-G R1 share 52aa sequence similarity and bind IFN-G in a species-specific manner.
IFN-G R1 is constitutively expressed in most cell types.
Soluble IFN-G R1 that binds IFN-G has been detected in biological fluids.
The recombinant soluble IFN-G R1 has been shown to bind IFN-G with high affinity and is a potent IFN-G antagonist.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-0.4ug/mlELISA (capture): 2-8ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile buffer.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, BSA. Reconstitute with sterile TBS, pH7.3 (20mM Trizma base, 150mM sodium chloride), 0.1% BSA to 200ug/ml. Labeled with Biotin.
Specificity：Recognizes mouse CD119.
Isotype：IgG
Calc Crossreactivity：Mo
Immunogen：Recombinant corresponding to aa26-253 from mouse CD119 expressed in NSO cells (Q91Y85).
