84-1195-57　［受注停止］ARG1 (Arginase 1, AI type I arginase, arginase 1 liver) (Carboxyfluorescein) 100Tests　A3340-14R

特徴

• Arginase 1 (ARG1) is a 35-40kD member of the arginase family of enzymes.
• It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages.
• ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly down-regulating NO synthase (NOS) activity by depriving this enzyme of its substrate.
• Human ARG1 is 322aa in length.
• Its enzyme region comprises aa9-309 and contains two Mn atoms.
• ARG1 is modestly active as a monomer, but highly active as a 105kD homotrimer.
• Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS.
• There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa204-289.
• Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.
• Applications:Suitable for use in Flow Cytometry.
• Other applications not tested.
• Recommended Dilution:Flow Cytometry (intracellular staining); Neat.
• Use 10ul per 10^6 cells
• Optimal dilutions to be determined by the researcher.
• Absorption: 488nm
• Emission: 515-545nm
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.
• CFS conjugates are sensitive to light.