84-1195-56　Ah Receptor (Aryl Hydrocarbon Receptor, AHR, Aromatic Hydrocarbon Receptor, BHLHE76, Class E Basic Helix-loop-helix Protein 76, HGNC:348) 100ug　A1059-72J

特徴

• AHR is a 100-105kD member of the bHLH/PAS transcription factor family.
• It is widely expressed and serves many functions.
• First, it binds multiple xenobiotic chemicals in the cytoplasm.
• This induces dimerization with ARNT, translocation to the nucleus, and activation of P450 genes such as CYP1A1 and UGT1A6.
• Second, it appears to block cell cycle progression, possibly via a downregulation of CDK proteins.
• And third, it blocks apoptosis by interacting with E2F1, thus silencing Tap73 and Apaf1 genes.
• Mouse AHR precursor is 848aa in length.
• It contains a nine aa prosegment, plus an 839aa mature molecule that contains a DNA binding motif (aa12-39), a bHLH region (aa40-80), two PAS domains (aa116-336) and one PAC segment that stabilizes the PAS domains (aa342-383).
• There are multiple alleles for mouse AHR.
• One 95-97kD allele shows a premature truncation after Ser805, while a second 112kD allele shows a 41aa substitution for aa843-848.
• Over aa706-805, mouse AHR shares 87-63aa identity with rat and human AHR, respectively.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.