Globular tubulin subunits comprise the microtubule building block, with a/b-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells.
As a critical part of the microtubule-organizing center (MTOC), the third member of the tubulin superfamily, y-tubulin, is required for microtubule nucleation as well as centrosome duplication and spindle assembly (1,2, reviewed in 3).
y-tubulin forms complexes of two different sizes: y-tubulin small complex (y-TuSC) and the larger y-tubulin ring complex (y-TuRC).
Each complex consists of a number of y-tubulin complex proteins (GCPs) with y-tubulin itself being considered GCP1.
GCP2-6 all share sequence similarity in 5 different regions and it is thought that these areas could play a role in the proper folding of the proteins (4).
y-TuSC is composed of two y-tubulin molecules as well as GCP2 and GCP3.
y-TuRC is made up of a ring of multiple copies of y-TuSC in addition to GCP4, 5, and 6.
Another protein, GCP-WD/NEDD1, which lacks sequence similarity with the other GCPs, associates with the y-TuRC.
GCP-WD/NEDD1 has been shown to regulate localization of the y-TuSC to spindles and centrosomes (5-8).
In mammals, phosphorylation of y-tubulin at Ser131 by SADB controls the activity of the y-TuRC.
The hypothesis is that this phosphorylation stabilizes the protein in a conformation that stimulates centrosome amplification (9).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human TUBG. Species Crossreactivity: Monkey, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to residues near the C-terminus of human TUBG.
