Succinate dehydrogenase (SDH), also known as Complex II or succinate:quinone oxidoreductase, is a key component of the citric acid cycle and the electron transport chain (1).
Specifically, it is involved in the oxidation of succinate (2).
SDH consists of four subunits: SDHA, SDHB, SDHC, and SDHD (3).
Defects in SDHA cause complex II deficiency (2).
In addition, reduction of SDHA has been observed in the striatum of patients with Huntington's Disease (3).
Reduction of SDHB, SDHC, and SDHD are often observed in paragangliomas and phenochromocytomas (4,5).
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence (IF-IC): 1:50Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity and peptide chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human SDHA. Species Crossreactivity: Monkey, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to residues near the C-terminus of human SDHA.
![SDHA (Succinate Dehydrogenase [ubiquinone] Flavoprotein Subunit, Mitochondrial, Flavoprotein Subunit of Complex II, Fp, SDH2, SDHF) 100ul](https://aimg.as-1.co.jp/c/84/1195/45/85133520.jpg)