RanBP3 was originally identified as RanGTP binding protein located in the nucleus and involved in the nuclear exporting process (1).
It functions as a cofactor for CRM1 nuclear export by binding to CRM1, stabilizing the RanGTP-CRM1-cargo interaction and promoting complex association with nuclear pore proteins (2,3).
In the absence of Ran-bound GTP, RanBP3 prevents binding of CRM1 complex to the nuclear pore complex.
In addition to CRM1, RanBP3 also has been shown to bind to RanGEF-RCC1 and increase the guanine nucleotide exchange activity of RCC1 for RanGTP-CRM1-Cargo (1,4).
In some cases, as with B-catenin and Smad2/3, RanBP3 binding may mediate the target protein nuclear export in a Ran-dependent, but CRM1-independent manner (5,6).
RanBP3 is phosphorylated at Ser58 through the PI3K/Akt or ERK/RSK pathway.
This phosphorylation is important for RanBP3 function in nuclear export, likely due to stimulation of RCC1 activity (7,8).
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human RanBP3b when phosphorylated at Ser58 and RanBP3a when phosphorylated at corresponding residue Ser126. Species Crossreactivity: monkey.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mk
Immunogen：Synthetic peptide corresponding to residues surrounding Ser58 of human RanBP3b.
