Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1).
IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth and susceptibility to transformation.
The IRF family includes nine members: IRF-1, IRF-2, ISGF3gamma/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7 and IRF-8/ICSBP.
All IRF proteins share homology in their amino-terminal DNA binding domains.
IRF family members regulate transcription through interactions with proteins that share similar DNA binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS) and IFN regulatory elements (IRF-E) (2).
While IRF family members generally function in innate immune responses, IRF-6 has not been associated in that role.
Original studies of IRF-6 found that mutation of the gene for IRF-6 caused Van der Woude Syndrome, an autosomal dominant disorder resulting in mouth abnormalities including cleft lip and palate (3).
IRF-6 knockouts show a hyperproliferative epidermis that fails to undergo kerotinocyte differentiation (4).
IRF-6 has also been found to interact with the mammary tumor suppressor maspin, and like maspin is expressed in normal mammary epithelial but reduced or absent in breast carcinoma (5).
Cellular proliferation may promote IRF-6 phosphorylation leading to its proteasomal dependent degradation (6).
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human IRF-6.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to residues surrounding Gly121 of human IRF-6. Species sequence homology: Canine, bovine and monkey (100%)
