Histone H2A.X is a variant histone that represents approximately 10% of the total H2A histone proteins in normal human fibroblasts (1).
H2A.X is required for checkpoint-mediated cell cycle arrest and DNA repair following double-stranded DNA breaks (1).
DNA damage, caused by ionizing radiation, UV-light, or radiomimetic agents, results in rapid phosphorylation of H2A.X at Ser139 by PI3K-like kinases, including ATM, ATR and DNA-PK (2,3).
Within minutes following DNA damage, H2A.X is phosphorylated on Ser139 at sites of DNA damage (4).
This very early event in the DNA-damage response is required for recruitment of a multitude of DNA-damage response proteins, including MDC1, NBS1, RAD50, MRE11, 53BP1 and BRCA1 (1).
In addition to its role in DNA-damage repair, H2A.X is required for DNA fragmentation during apoptosis and is phosphorylated by various kinases in response to apoptotic signals.
H2A.X is phosphorylated on Ser139 by DNA-PK in response to cell death receptor activation, c-Jun N-terminal Kinase (JNK1) in response to UV-A irradiation, and p38 MAPK in response to serum starvation (5-8).
H2A.X is constitutively phosphorylated on Tyr142 in undamaged cells by WSTF (Williams-Beuren syndrome transcription factor) (9,10).
Applications:Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Immunofluorescence (IC): 1:100 Flow Cytometry: 1:200Western Blot: 1:1000 Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human histone H2A.X, phosphorylated at Ser139 or Tyr142, or dually phosphorylated on both residues. Does not react with histone proteins. Other applications have not been tested. Species Crossreactivity: monkey, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：FC IF IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding the phosphorylated Ser139 and Tyr142 of human H2A.X.
