84-1195-30　DLST (Dihydrolipoyllysine-residue Succinyltransferase Component Of 2-oxoglutarate Dehydrogenase Complex, Mitochondrial, Dihydrolipoamide Succinyltransferase Component Of 2-oxoglutarate Dehydrogenase Complex, E2, E2K, DLTS) 100ul　D7800-20C

特徴

• Alpha-ketoglutarate dehydrogenase complex is a rate-regulating enzyme in the Krebs Cycle in mitochondria.
• Dihydrolipoamide succinyltransferase (DLST) is a key subunit in this complex.
• Mutations in the DLST gene may be responsible for the familial Alzheimer disease.
• Reduction of DLST increases the generation of reactive oxygen species production suggesting its role in oxidative stress.
• Deficiency of DLST in mice was suggested to cause increased oxidative stress in mitochondria and is involved in the pathogenesis of Alzheimer disease.
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000 incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
• Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap