Deleted in breast cancer gene 1 protein (DBC1) was originally identified by its localization to a region of chromosome 8p21 that is homozygously deleted in breast cancer (1).
DBC1 is a large, nuclear protein with multiple functions in cell survival.
It binds directly to the estrogen receptor a (ERa) hormone-binding domain in a ligand-independent manner and may be a key determinant of ligand-independent ERa expression and survival in human breast cancer cells (2).
DBC1 can promote p53-mediated apoptosis by binding to and inhibiting the deacetylase activity of SirT1, resulting in increased p53 acetylation levels and activity (3).
DBC1 may be an important regulator of heterochromatin formation as it binds SUV39H1 and inhibits its histone methyltransferase activity (4).
Caspase-dependent processing activates the pro-apoptotic activity of DBC1 during Tumor Necrosis Factor-a (TNF-a)-mediated cell death signaling (5).
This processing of DBC1 in response to TNF-a is an early event in the onset of apoptosis and results in relocalization of DBC1 to the cytoplasm.
Over-expression of the processed, cytoplasmic form of DBC1 results in mitochondrial clustering and matrix condensation and sensitizes cells to TNF-a-mediated apoptosis.
Applications:Suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunoprecipitation: 1:50Immunofluorescence (IF-IC): 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human DBC1. Species Crossreactivity: monkey, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IF IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to residues near the N-terminus of human DBC1. Species sequence homology: equine (100%)
