MEF2A (myocyte specific enhancer factor 2) is a transcription factor protein that belongs to MEF2 family, a family of DNA binding regulatory proteins.
MEF2 family members (MEF2A, 2B, 2C, and 2D) are highly expressed during dendritic maturation and synapse formation in the brain and are involved in the regulation of development and function of T-cell, muscle cells, and brain cells.
Highly expressed during synaptogenesis, MEF2A binds to MEF2 element present in the regulatory regions in various muscle specific genes and activates transcription.
MEF2A has been implicated in cardiac and skeletal muscle development.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:200Western Blot: 1:500-1:1000 Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human MEF2A only when phosphorylated at Thr312. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to the area near the phosphorylation site of threonine 312 (L-A-pT-P-V) of human MEF2A.
