84-1195-20　ZBTB10 (Zinc Finger and BTB Domain-containing Protein 10, FLJ12752, RINZF, RINZFC, Zinc Finger Protein RIN ZF) 100ug　Z0331

特徴

• RIN zinc finger protein (RINZF):ZBTB10 is a novel zinc finger protein that inhibits Sp1-dependent activation.
• In this regard, ZBTB10 has been identified as a putative suppressor of Sp proteins such as Sp1, Sp3 and Sp4.
• Sp proteins belong to the Sp:Kruppel-like factor (KLF) family of transcription factors and are overexpressed in certain cancers.
• Overexpression of Sp proteins is thought to contribute to the proliferative and angiogenic phenotype associated with cancer cells.
• Although the underlying factors associated with high expression of Sp proteins is not well understood, it is thought that microRNAs (miRNA) may play a role in mediating their overexpression in cancer cells.
• Like Sp proteins, miRNA-27a (miR-27a) is widely expressed in certain cancer cells.
• MiR-27a suppresses ZB1B10 expression and suppression of ZBTB10 by overexpressed miR-27a may be linked to overexpression of Sp proteins in breast cancer cells (Mertens-Talcott et al, 2007).
• These authors showed that introduction of antisense miR-27a resulted in increased expression of ZBTB10 mRNA and decreased expression of Sp1, Sp3 and Sp4 mRNA and protein.
• It is thought that the oncogenic activity of miR-27a may be due, in part, to suppression of ZBTB10 which leads to increased expression of Sp proteins thereby contributing to the proliferative and angiogenic cancer phenotype.
• g 10, RINZF is a novel member of the Cys2-His2 zinc finger gene family, having a cDNA sequence of 3.8 kilobases.
• Apart from the Cys2-His2 zinc finger domains, the RIN ZF coding sequence bears little resemblance to other reported zinc finger genes.
• The amino-terminal region of the protein is an activation domain, despite the lack of recognizable activation motifs, such as homopolymeric glutamine and proline-rich stretches.
• Instead, it possesses several regions rich in basic residues, often associated with repressor domains.
• The gene encoding the RINZF protein is mapped on human chromosome 8q13-q21.1.
• The protein is highly conserved and acts as a transcriptional regulator.
• The protein competes with Sp1 binding at the CACC cis-regulatory element of the gastrin promoter and thus has a down-regulatory effect on gastrin transcription.
• The existence of two transcript variants, isoform 1 and 2, produced by alternate splicing of the RINZF gene has been studied.
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.