ZBTB2 is a member of the POK (POZ domain Krüppel-like zinc finger) family and plays an important role in cell cycle regulation.
It is a transcription factor and a potent repressor of the ARF-HDM2-p53-p21 pathway.
ZBTB2 represses the transcription of the p21, p53 and ARF genes and activates HDM2 gene.
ZBTB2 and Sp1 compete with each other in binding to the GC-box 5:6 elements and the two p53 binding elements.
ZBTB2 directly interacted with p53 via its zinc fingers, inhibiting p53 binding and repressing transcription activation by p53.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 2-5ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human ZBTB2. Species Crossreactivity: canine, equine, monkey, mouse, opossum, rat. Species sequence homology: chimpanzee, bovine.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Ca Eq Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to aa450 to 500 of human ZBTB2.
