XIAP [human X-linked IAP, hILP (human IAP-like protein), MIHA, BIRC4) is a member of the family of inhibitor of apoptosis proteins (IAP).
IAPs suppress mitochondria-dependent and -independent apoptosis by binding to and inhibiting caspases through their BIR domains (reviewed in Liston et al, 2003; Wright and Duckett, 2005).
Resistance towards apoptosis is a hallmark of cancer cells, and overexpression of IAPs can contribute to the development of cancer though inhibiting apoptosis.
In addition to at least one BIR domain, some IAP members also have a RING-type finger motif at their carboxyl-terminal.
The RING finger domain of several IAPs, including XIAP, have E3 ubiquitin ligase activity and target the degradation of Smac/DIABLO through ubqiuitination (Morizane et al, 2005).
Smac/DIABLO is a death inducer and functions by inhibiting IAP-caspase interactions, thereby promoting apoptosis.
Degradation of cell death inducers like Smac/DIABLO is thought to be a conserved mechanism by which IAPs enhance their anti-apoptotic activity, thereby promoting cell survival.
XIAP is highly characterized with respect to its structure and biochemical mechanisms, and has received interest as a therapeutic target (reviewed in Schimmer, 2006).
Since XIAP blocks a substantial portion of the apoptosis pathway and is associated with chemoresistance in cancer cells, inhibiting XIAP has been a focus for potential therapeutics.
Approaches have included antisense oligonucleotides and small molecule inhibitors.
Small molecules that that target the BIR2 and BIR3 domains of XIAP are considered particularly attractive.
This is because the BIR domains inhibit caspase activity, and it is thought that removing the inhibition should increase the cell's ability to undergo apoptosis as well as decrease its potential for chemoresistance.
Recognizes XIAP.
Full-length human XIAP is a 497aa protein and migrates at ~53kD on SDS-PAGE.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:2000Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000Immunoprecipitation: 1:50-1:200Immunohistochemistry: FrozenOptimal dilutions to be determined by the researcher.
Positive Control:Spleen, lymphatic tissues, prostate, colon, many cancer cell lines
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide.
Specificity：Recognizes human XIAP. Species Crossreactivity: gerbil, mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant corresponding to BIR2 domain protein fragment of human XIAP. The BIR2 domain used for corresponds aa163-230 of human XIAP (Deveraux et al, 1999).
