Wnt5A is a nontransforming member of the Wnt family of secreted and cysteine-rich proteins that exert their cellular effects via autocrine or paracrine routes.
It is a proto-oncogene that functions in modulating cell fate and cell behavior during vertebrate development and has an important role in the Wnt signaling pathway.
It acts as a ligand for members of the frizzled family of seven transmembrane receptors.
The protein also plays a role in preventing metastatic disease in invasive breast carcinomas.
The protein has many conserved features like a hydrophobic signal sequence, Wnt1 family signature sequence (CKCHGvSGSC), and a number of other conserved aa residues.
Human Wnt5A expression was detected only in neonatal heart and lung.
The gene for human Wnt5A is localized on chromosome 3p21-p14.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human Wnt5A. Species Crossreactivity: bovine, monkey, mouse, rat. Species sequence homology: chimpanzee, pufferfish, xenopus, opossum.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to aa195-211 (EFVDARERERIHAKGSY) of human Wnt5A, (NP_003383).
