A novel member of the vesicle-associated membrane protein (VAMP):synaptobrevin family, VAMP-3 is a v-SNARE protein that resides in recycling endosomes and endosome-derived transport vesicles.
It colocalizes with endocytosed transferrin receptors and the glucose transporter GLUT4 in adipocytes, and is also present in alpha-granules in platelets.
The protein has been implicated in recycling of transferrin receptors to the plasma membrane, secretion of alpha-granules in platelets, recycling of T-cell receptors to the immunological synapses, and membrane trafficking during cell migration.
It is a homologue of VAMP-2 and has a broad tissue distribution in humans.
Like VAMP-2, it can be cleaved by tetanus toxin, a metallo-endoprotease which blocks synaptic exocytosis.
In humans, the VAMP-3 gene is mapped to chromosome 1p36.23.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human, mouse and rat brain lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS containing 0.05% BSA and 0.05% sodium azide
Specificity：Recognizes human VAMP-3. Species Crossreactivity: mouse and rat. Species sequence homology: New World monkey
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to a portion of amino acids 1-50 of human VAMP-3
