VAMP-2 is a member of the vesicle-associated membrane protein (VAMP):synaptobrevin family, which are 18kD integral membrane proteins originally characterized in synaptic vesicles.
The protein forms a stable complex with syntaxin, synaptosomal-associated protein and synaptotagmin which are main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane.
VAMP-2 plays a key role in the fusion of synaptic vesicles.
VAMP-2 is also expressed in non-neuronal tissue and participates in regulated exocytosis, such as insulin-regulated translocation of glucose transporter-4 (GLUT4)-containing vesicles in adipocytes and insulin secretion in pancreatic beta-cells.
In human, the pathological role of the protein has been identified in familial infantile myasthenia (FIMG).
The rat VAMP-2 gene is localized on chromosome 10q24.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Brain
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes rat VAMP-2. Species Crossreactivity: mouse. Species sequence homology: human.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo Rt
Immunogen：Synthetic peptide corresponding to aa1-50 of rat VAMP-2.
