UBE1L2 is a novel E1 enzyme specific for ubiquitin and belongs to the ubiquitin-activating E1 family.
It is a part of the cascade to attach ubiquitin (Ub) covalently to substrate proteins.
It activates ubiquitin by first adenylating its carboxy-terminal glycine residue with ATP and then linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
Its isoforms influence spermatogenesis and male fertility.
It is highly expressed in human monocytes.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-3ug/mlImmunohistochemistry (paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Daudi and RAW cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human UBE1L2/MOP-4. Species Crossreactivity: chimpanzee. Species sequence homology: bovine, equine, mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa300-350 of human UBE1L2/MOP-4.
