TRADD [tumor necrosis factor receptor type 1-associated (TNFR1) death domain protein] is a death domain containing adaptor molecule that interacts with TNFR1:TNFRSF1A and mediates programmed cell death signaling and NF-kB activation (reviewed in Feng, 2005).
TRADD binds adaptor protein TRAF2, reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thereby suppresses TRAF2 mediated apoptosis.
TRADD can also interact with receptor TNFRSF6:FAS and adaptor protein FADD:MORT1, and is involved in the Fas-induced cell death pathway.
Recognizes TRADD; human TRADD is a 312aa protein.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:2000Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000Immunoprecipitation: 1:50-1:200Immunohistochemistry: FrozenOptimal dilutions to be determined by the researcher.
Positive Control:Spleen, lymphoid, many cancer cell lines
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide.
Specificity：Recognizes human TRADD.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa172-192 (DGEVASAPLQPPVPSLSEVKP) of human TRADD; GenBank no. gi|14970570|emb|CAC38018.2|
