TRIADs are novel zinc finger proteins characterized by the presence of Cysteine rich TRIAD domain consisting of DRIL motif flanked by 2 RING fingers.
TRIAD3, a 56kD protein, is a novel member of this family.
It contains 4 RLD domains in the centre and a proline-rich domain at the C-terminus.
Localized in the cytoplasm, it interacts with RIP and inhibits TNF and IL1-induced NF-kappaB activation pathways.
Reports suggest TRIAD3 acts as an E3-ubiquitin-protein ligase and promotes ubiquitination and degradation of TLR4 and TLR9, thereby regulating TLR signaling.
It also interacts with HIV type I Vif protein and interferes in the early events of HIV-1 replication.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human brain, heart, kidney, liver, lung, ovary, pancreas, small intestine, skeletal muscle, spleen, or testes.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity Chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human TRIAD3A.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to a portion of human TRIAD3 between aa350-400.
